Review



mcd medium  (Welgene inc)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Welgene inc mcd medium
    Mcd Medium, supplied by Welgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcd+medium/mcdb+131+medium/pmc05931608-215-38-40
    Average 90 stars, based on 1 article reviews
    mcd medium - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    other:

    Article Title: Succinate causes α-SMA production through GPR91 activation in hepatic stellate cells.
    Article Snippet: Succinate acts as an extracellular signaling molecule as well as an intermediate in the citric acid cycle.. It binds to and activates its specific G protein-coupled receptor 91 (GPR91).. GPR91 is present in hepatic stellate cells (HSCs), but its role in hepatic fibrogenesis remains unclear.

    Article Title: Sirtuin 3 (SIRT3) Regulates α-Smooth Muscle Actin (α-SMA) Production through the Succinate Dehydrogenase-G Protein-coupled Receptor 91 (GPR91) Pathway in Hepatic Stellate Cells
    Article Snippet: DMEM completely deficient in methionine and choline (MCD medium) and a methionine and choline supplement (MCS medium, control medium) were purchased from Welgene (Kyeongsan, Korea).

    Expressing:

    Article Title: Growth differentiation factor 15 ameliorates nonalcoholic steatohepatitis and related metabolic disorders in mice
    Article Snippet: .. For analysis of GDF15 expression in an in vitro NASH model, Hepa1c1c7, KUP5 and hTERT-HSC cells were seeded at 6-well plates, incubated for 24 h and maintained in serum-free DMEM for 24 h. Cells were then incubated in MCD medium (WelGENE Inc.) or control DMEM for 18 h. To analyze fibrotic gene expression, serum-starved hTERT-HSCs and primary mouse HSCs were pre-treated with recombinant GDF15 (0.3, 1, 50 or 100 ng/ml) for 30 min, and then were incubated with recombinant TGFβ1 (2.5 ng/ml) for 18 h. .. Total RNA was isolated from various cells or liver tissues using Hybrid-R or Ribospin TM II Kit (GeneAll Biotechnology) according to the manufacturer’s instruction. cDNA was synthesized from 2 μg of total RNA using Moloney Murine Leukemia Virus (MMLV)-reverse transcriptase (Promega) and oligo(T) primer at 42 °C for 1 h. An aliquot (1/160 vol) of the cDNA was then subjected to PCR amplification using mouse or human gene-specific primers (Supplementary Table ).

    In Vitro:

    Article Title: Growth differentiation factor 15 ameliorates nonalcoholic steatohepatitis and related metabolic disorders in mice
    Article Snippet: .. For analysis of GDF15 expression in an in vitro NASH model, Hepa1c1c7, KUP5 and hTERT-HSC cells were seeded at 6-well plates, incubated for 24 h and maintained in serum-free DMEM for 24 h. Cells were then incubated in MCD medium (WelGENE Inc.) or control DMEM for 18 h. To analyze fibrotic gene expression, serum-starved hTERT-HSCs and primary mouse HSCs were pre-treated with recombinant GDF15 (0.3, 1, 50 or 100 ng/ml) for 30 min, and then were incubated with recombinant TGFβ1 (2.5 ng/ml) for 18 h. .. Total RNA was isolated from various cells or liver tissues using Hybrid-R or Ribospin TM II Kit (GeneAll Biotechnology) according to the manufacturer’s instruction. cDNA was synthesized from 2 μg of total RNA using Moloney Murine Leukemia Virus (MMLV)-reverse transcriptase (Promega) and oligo(T) primer at 42 °C for 1 h. An aliquot (1/160 vol) of the cDNA was then subjected to PCR amplification using mouse or human gene-specific primers (Supplementary Table ).

    Incubation:

    Article Title: Growth differentiation factor 15 ameliorates nonalcoholic steatohepatitis and related metabolic disorders in mice
    Article Snippet: .. For analysis of GDF15 expression in an in vitro NASH model, Hepa1c1c7, KUP5 and hTERT-HSC cells were seeded at 6-well plates, incubated for 24 h and maintained in serum-free DMEM for 24 h. Cells were then incubated in MCD medium (WelGENE Inc.) or control DMEM for 18 h. To analyze fibrotic gene expression, serum-starved hTERT-HSCs and primary mouse HSCs were pre-treated with recombinant GDF15 (0.3, 1, 50 or 100 ng/ml) for 30 min, and then were incubated with recombinant TGFβ1 (2.5 ng/ml) for 18 h. .. Total RNA was isolated from various cells or liver tissues using Hybrid-R or Ribospin TM II Kit (GeneAll Biotechnology) according to the manufacturer’s instruction. cDNA was synthesized from 2 μg of total RNA using Moloney Murine Leukemia Virus (MMLV)-reverse transcriptase (Promega) and oligo(T) primer at 42 °C for 1 h. An aliquot (1/160 vol) of the cDNA was then subjected to PCR amplification using mouse or human gene-specific primers (Supplementary Table ).

    Control:

    Article Title: Growth differentiation factor 15 ameliorates nonalcoholic steatohepatitis and related metabolic disorders in mice
    Article Snippet: .. For analysis of GDF15 expression in an in vitro NASH model, Hepa1c1c7, KUP5 and hTERT-HSC cells were seeded at 6-well plates, incubated for 24 h and maintained in serum-free DMEM for 24 h. Cells were then incubated in MCD medium (WelGENE Inc.) or control DMEM for 18 h. To analyze fibrotic gene expression, serum-starved hTERT-HSCs and primary mouse HSCs were pre-treated with recombinant GDF15 (0.3, 1, 50 or 100 ng/ml) for 30 min, and then were incubated with recombinant TGFβ1 (2.5 ng/ml) for 18 h. .. Total RNA was isolated from various cells or liver tissues using Hybrid-R or Ribospin TM II Kit (GeneAll Biotechnology) according to the manufacturer’s instruction. cDNA was synthesized from 2 μg of total RNA using Moloney Murine Leukemia Virus (MMLV)-reverse transcriptase (Promega) and oligo(T) primer at 42 °C for 1 h. An aliquot (1/160 vol) of the cDNA was then subjected to PCR amplification using mouse or human gene-specific primers (Supplementary Table ).

    Gene Expression:

    Article Title: Growth differentiation factor 15 ameliorates nonalcoholic steatohepatitis and related metabolic disorders in mice
    Article Snippet: .. For analysis of GDF15 expression in an in vitro NASH model, Hepa1c1c7, KUP5 and hTERT-HSC cells were seeded at 6-well plates, incubated for 24 h and maintained in serum-free DMEM for 24 h. Cells were then incubated in MCD medium (WelGENE Inc.) or control DMEM for 18 h. To analyze fibrotic gene expression, serum-starved hTERT-HSCs and primary mouse HSCs were pre-treated with recombinant GDF15 (0.3, 1, 50 or 100 ng/ml) for 30 min, and then were incubated with recombinant TGFβ1 (2.5 ng/ml) for 18 h. .. Total RNA was isolated from various cells or liver tissues using Hybrid-R or Ribospin TM II Kit (GeneAll Biotechnology) according to the manufacturer’s instruction. cDNA was synthesized from 2 μg of total RNA using Moloney Murine Leukemia Virus (MMLV)-reverse transcriptase (Promega) and oligo(T) primer at 42 °C for 1 h. An aliquot (1/160 vol) of the cDNA was then subjected to PCR amplification using mouse or human gene-specific primers (Supplementary Table ).

    Recombinant:

    Article Title: Growth differentiation factor 15 ameliorates nonalcoholic steatohepatitis and related metabolic disorders in mice
    Article Snippet: .. For analysis of GDF15 expression in an in vitro NASH model, Hepa1c1c7, KUP5 and hTERT-HSC cells were seeded at 6-well plates, incubated for 24 h and maintained in serum-free DMEM for 24 h. Cells were then incubated in MCD medium (WelGENE Inc.) or control DMEM for 18 h. To analyze fibrotic gene expression, serum-starved hTERT-HSCs and primary mouse HSCs were pre-treated with recombinant GDF15 (0.3, 1, 50 or 100 ng/ml) for 30 min, and then were incubated with recombinant TGFβ1 (2.5 ng/ml) for 18 h. .. Total RNA was isolated from various cells or liver tissues using Hybrid-R or Ribospin TM II Kit (GeneAll Biotechnology) according to the manufacturer’s instruction. cDNA was synthesized from 2 μg of total RNA using Moloney Murine Leukemia Virus (MMLV)-reverse transcriptase (Promega) and oligo(T) primer at 42 °C for 1 h. An aliquot (1/160 vol) of the cDNA was then subjected to PCR amplification using mouse or human gene-specific primers (Supplementary Table ).



    Similar Products

    mcd medium  (Thermo Fisher)
    90
    Thermo Fisher mcd medium
    Mcd Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcd+medium/pm28066964-71-23-25
    Average 90 stars, based on 1 article reviews
    mcd medium - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    methioninecholine deficient (mcd) medium  (Thermo Fisher)
    90
    Thermo Fisher methioninecholine deficient (mcd) medium
    Methioninecholine Deficient (Mcd) Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcd+medium/pm35978143-246-21-25
    Average 90 stars, based on 1 article reviews
    methioninecholine deficient (mcd) medium - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    methionine choline deficient mcd medium  (Thermo Fisher)
    86
    Thermo Fisher methionine choline deficient mcd medium
    a Western blot showing the modulation of ACE2 and NRP1 protein expression when incubated with methionine-choline deficient <t>(MCD)</t> medium for 6 and 24 h (along with their respective controls. Quantification of ACE2 or NRP1 protein expression by densitometry is also shown ( n = 3, unpaired t -test). b , c Western blot showing the expression of ACE2 and NRP1 protein expression when treated with 1 mM metformin for 6 or 24 h ( n = 3, unpaired t -test) ( b ) or with MCD or MCD plus 1 mM metformin for 10 h ( n = 3, one-way ANOVA) ( c ) as well as their respective controls. Relative quantification of ACE2 or NRP1 protein expression (related to β-actin) is also shown. d Relative infection with pseudotyped viral particles or controls of WT or hACE2 mouse hepatocytes (left) or upcyte second-generation human hepatocytes (right) upon treatment with MCD or MCD and metformin, measured by flow cytometry (one-way ANOVA). e Extracellular levels of ANG(1–7) secreted by human primary hepatocytes after infection with pseudotyped viral particles upon treatment with MCD or MCD and metformin, measured by ELISA ( n = 5, one-way ANOVA). Error bars represent SEM and asterisks represent p values (*<0.05, **<0.01, and ***<0.001).
    Methionine Choline Deficient Mcd Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcd+medium/pmc09383691-225-21-25
    Average 86 stars, based on 1 article reviews
    methionine choline deficient mcd medium - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    mcd-mimicking medium  (US Biological Life Sciences)
    90
    US Biological Life Sciences mcd-mimicking medium
    a Western blot showing the modulation of ACE2 and NRP1 protein expression when incubated with methionine-choline deficient <t>(MCD)</t> medium for 6 and 24 h (along with their respective controls. Quantification of ACE2 or NRP1 protein expression by densitometry is also shown ( n = 3, unpaired t -test). b , c Western blot showing the expression of ACE2 and NRP1 protein expression when treated with 1 mM metformin for 6 or 24 h ( n = 3, unpaired t -test) ( b ) or with MCD or MCD plus 1 mM metformin for 10 h ( n = 3, one-way ANOVA) ( c ) as well as their respective controls. Relative quantification of ACE2 or NRP1 protein expression (related to β-actin) is also shown. d Relative infection with pseudotyped viral particles or controls of WT or hACE2 mouse hepatocytes (left) or upcyte second-generation human hepatocytes (right) upon treatment with MCD or MCD and metformin, measured by flow cytometry (one-way ANOVA). e Extracellular levels of ANG(1–7) secreted by human primary hepatocytes after infection with pseudotyped viral particles upon treatment with MCD or MCD and metformin, measured by ELISA ( n = 5, one-way ANOVA). Error bars represent SEM and asterisks represent p values (*<0.05, **<0.01, and ***<0.001).
    Mcd Mimicking Medium, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcd+medium/mcd+mimicking+medium/pm35568098-64-0-6
    Average 90 stars, based on 1 article reviews
    mcd-mimicking medium - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    mcd medium  (Difco)
    90
    Difco mcd medium
    a Western blot showing the modulation of ACE2 and NRP1 protein expression when incubated with methionine-choline deficient <t>(MCD)</t> medium for 6 and 24 h (along with their respective controls. Quantification of ACE2 or NRP1 protein expression by densitometry is also shown ( n = 3, unpaired t -test). b , c Western blot showing the expression of ACE2 and NRP1 protein expression when treated with 1 mM metformin for 6 or 24 h ( n = 3, unpaired t -test) ( b ) or with MCD or MCD plus 1 mM metformin for 10 h ( n = 3, one-way ANOVA) ( c ) as well as their respective controls. Relative quantification of ACE2 or NRP1 protein expression (related to β-actin) is also shown. d Relative infection with pseudotyped viral particles or controls of WT or hACE2 mouse hepatocytes (left) or upcyte second-generation human hepatocytes (right) upon treatment with MCD or MCD and metformin, measured by flow cytometry (one-way ANOVA). e Extracellular levels of ANG(1–7) secreted by human primary hepatocytes after infection with pseudotyped viral particles upon treatment with MCD or MCD and metformin, measured by ELISA ( n = 5, one-way ANOVA). Error bars represent SEM and asterisks represent p values (*<0.05, **<0.01, and ***<0.001).
    Mcd Medium, supplied by Difco, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcd+medium/yeast+nitrogen+base+without+amino+acids/pmc06504898-165-5-14
    Average 90 stars, based on 1 article reviews
    mcd medium - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    mcd medium  (Welgene inc)
    90
    Welgene inc mcd medium
    a Western blot showing the modulation of ACE2 and NRP1 protein expression when incubated with methionine-choline deficient <t>(MCD)</t> medium for 6 and 24 h (along with their respective controls. Quantification of ACE2 or NRP1 protein expression by densitometry is also shown ( n = 3, unpaired t -test). b , c Western blot showing the expression of ACE2 and NRP1 protein expression when treated with 1 mM metformin for 6 or 24 h ( n = 3, unpaired t -test) ( b ) or with MCD or MCD plus 1 mM metformin for 10 h ( n = 3, one-way ANOVA) ( c ) as well as their respective controls. Relative quantification of ACE2 or NRP1 protein expression (related to β-actin) is also shown. d Relative infection with pseudotyped viral particles or controls of WT or hACE2 mouse hepatocytes (left) or upcyte second-generation human hepatocytes (right) upon treatment with MCD or MCD and metformin, measured by flow cytometry (one-way ANOVA). e Extracellular levels of ANG(1–7) secreted by human primary hepatocytes after infection with pseudotyped viral particles upon treatment with MCD or MCD and metformin, measured by ELISA ( n = 5, one-way ANOVA). Error bars represent SEM and asterisks represent p values (*<0.05, **<0.01, and ***<0.001).
    Mcd Medium, supplied by Welgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcd+medium/mcdb+131+medium/pmc05931608-215-38-40
    Average 90 stars, based on 1 article reviews
    mcd medium - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    mcd-131 medium supplemental microvascular growth supplement  (Thermo Fisher)
    90
    Thermo Fisher mcd-131 medium supplemental microvascular growth supplement
    a Western blot showing the modulation of ACE2 and NRP1 protein expression when incubated with methionine-choline deficient <t>(MCD)</t> medium for 6 and 24 h (along with their respective controls. Quantification of ACE2 or NRP1 protein expression by densitometry is also shown ( n = 3, unpaired t -test). b , c Western blot showing the expression of ACE2 and NRP1 protein expression when treated with 1 mM metformin for 6 or 24 h ( n = 3, unpaired t -test) ( b ) or with MCD or MCD plus 1 mM metformin for 10 h ( n = 3, one-way ANOVA) ( c ) as well as their respective controls. Relative quantification of ACE2 or NRP1 protein expression (related to β-actin) is also shown. d Relative infection with pseudotyped viral particles or controls of WT or hACE2 mouse hepatocytes (left) or upcyte second-generation human hepatocytes (right) upon treatment with MCD or MCD and metformin, measured by flow cytometry (one-way ANOVA). e Extracellular levels of ANG(1–7) secreted by human primary hepatocytes after infection with pseudotyped viral particles upon treatment with MCD or MCD and metformin, measured by ELISA ( n = 5, one-way ANOVA). Error bars represent SEM and asterisks represent p values (*<0.05, **<0.01, and ***<0.001).
    Mcd 131 Medium Supplemental Microvascular Growth Supplement, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcd+medium/pmc05378165-499-9-16
    Average 90 stars, based on 1 article reviews
    mcd-131 medium supplemental microvascular growth supplement - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    a Western blot showing the modulation of ACE2 and NRP1 protein expression when incubated with methionine-choline deficient (MCD) medium for 6 and 24 h (along with their respective controls. Quantification of ACE2 or NRP1 protein expression by densitometry is also shown ( n = 3, unpaired t -test). b , c Western blot showing the expression of ACE2 and NRP1 protein expression when treated with 1 mM metformin for 6 or 24 h ( n = 3, unpaired t -test) ( b ) or with MCD or MCD plus 1 mM metformin for 10 h ( n = 3, one-way ANOVA) ( c ) as well as their respective controls. Relative quantification of ACE2 or NRP1 protein expression (related to β-actin) is also shown. d Relative infection with pseudotyped viral particles or controls of WT or hACE2 mouse hepatocytes (left) or upcyte second-generation human hepatocytes (right) upon treatment with MCD or MCD and metformin, measured by flow cytometry (one-way ANOVA). e Extracellular levels of ANG(1–7) secreted by human primary hepatocytes after infection with pseudotyped viral particles upon treatment with MCD or MCD and metformin, measured by ELISA ( n = 5, one-way ANOVA). Error bars represent SEM and asterisks represent p values (*<0.05, **<0.01, and ***<0.001).

    Journal: Communications Biology

    Article Title: The spike of SARS-CoV-2 promotes metabolic rewiring in hepatocytes

    doi: 10.1038/s42003-022-03789-9

    Figure Lengend Snippet: a Western blot showing the modulation of ACE2 and NRP1 protein expression when incubated with methionine-choline deficient (MCD) medium for 6 and 24 h (along with their respective controls. Quantification of ACE2 or NRP1 protein expression by densitometry is also shown ( n = 3, unpaired t -test). b , c Western blot showing the expression of ACE2 and NRP1 protein expression when treated with 1 mM metformin for 6 or 24 h ( n = 3, unpaired t -test) ( b ) or with MCD or MCD plus 1 mM metformin for 10 h ( n = 3, one-way ANOVA) ( c ) as well as their respective controls. Relative quantification of ACE2 or NRP1 protein expression (related to β-actin) is also shown. d Relative infection with pseudotyped viral particles or controls of WT or hACE2 mouse hepatocytes (left) or upcyte second-generation human hepatocytes (right) upon treatment with MCD or MCD and metformin, measured by flow cytometry (one-way ANOVA). e Extracellular levels of ANG(1–7) secreted by human primary hepatocytes after infection with pseudotyped viral particles upon treatment with MCD or MCD and metformin, measured by ELISA ( n = 5, one-way ANOVA). Error bars represent SEM and asterisks represent p values (*<0.05, **<0.01, and ***<0.001).

    Article Snippet: Steatotic conditions were induced in primary hepatocytes as follows: incubation overnight on 0%-FBS MEM medium containing PSG followed by incubation in methionine-choline deficient (MCD) medium (Gibco Cat#: ME120128L1) supplemented with PSG.

    Techniques: Western Blot, Expressing, Incubation, Infection, Flow Cytometry, Enzyme-linked Immunosorbent Assay